Contract type: Permanent
We are currently seeking an experienced molecular biologist to join the Protein Engineering group working on GPCR structure determination to enable drug discovery efforts.
The successful candidate will be expected to work independently, and the role will include the following responsibilities:
- Cloning of plasmid constructs for expression;
- Expression of GPCRs in insect, yeast and human cells;
- Characterization of GPCRs in membranes and in detergent-solubilised form using ligand binding assays;
- Establishing and optimising thermal stability assays;
- Mutant library generation and directed evolution of proteins;
- Screening for stabilising mutations using our proprietary platform to create StaR® proteins;
- Construct engineering to optimise expression;
- Purification and biochemical characterisation of GPCRs using a range of techniques such as size exclusion chromatography;
- Careful documentation of experimental work;
- Work closely with molecular biologists, protein expression scientists, biophysicists, structural biologists, and pharmacologists to help advance the project.
This is an exceptional opportunity to participate in pioneering science with an Industry-leading Drug Discovery company.
Our preferred candidate will have the following profile:
- PhD in a relevant area of biology
- Ability to work flexibly within a team, whilst being highly self-motivated and able to work independently;
- Analytical thinking and good problem-solving skills;
- Excellent organisational and time-management skills, including the ability to work to strict deadlines;
- Fluency in oral and written English is essential; and
- Relevant lab experience would be preferred including:
o Experience in a variety of molecular biology cloning techniques;
o Proficient in cell culture techniques for the expression of membrane proteins in mammalian, insect and yeast cells;
o Experience in membrane protein purification;
o Experience of assay development and optimisation;
o Experience in flow cytometry;
o Experience in GPCR in vitro pharmacology and GPCR biochemical assays would be desirable.
Application deadline: 31 May 2021